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extra thick fi lter paper  (Bio-Rad)


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    Bio-Rad extra thick fi lter paper
    Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi <t>lter.</t> The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.
    Extra Thick Fi Lter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extra thick fi lter paper/product/Bio-Rad
    Average 94 stars, based on 416 article reviews
    extra thick fi lter paper - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Antigen Processing"

    Article Title: Antigen Processing

    Journal: Methods in Molecular Biology

    doi: 10.1007/978-1-62703-218-6

    Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi lter. The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.
    Figure Legend Snippet: Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi lter. The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.

    Techniques Used: Expressing, Activity Assay



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    extra thick fi lter paper  (Bio-Rad)
    94
    Bio-Rad extra thick fi lter paper
    Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi <t>lter.</t> The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.
    Extra Thick Fi Lter Paper, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extra thick fi lter paper/product/Bio-Rad
    Average 94 stars, based on 1 article reviews
    extra thick fi lter paper - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi lter. The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.

    Journal: Methods in Molecular Biology

    Article Title: Antigen Processing

    doi: 10.1007/978-1-62703-218-6

    Figure Lengend Snippet: Fig. 1. Biochemical analysis of naturally processed peptides. Cells are homogenized in acid and fi ltered through a 3–30 kDa molecular fi lter. The fi ltrate containing the complex peptide mixture is then fractionated by reverse phase HPLC. The fractions, collected in 96-well plates, are dried and resuspended in buffer. Appropriate APC and T cells are added to the wells and after overnight culture, the T cell response is measured by the conversion of the lacZ substrate to a colored product. In the schematic shown, the naturally processed peptide in extracts of OVA expressing cells co-elutes with the synthetic SIINFEKL (SL8) peptide. Mock injections of buffer alone are carried out prior to each run to ensure that the activity in the test fractions is not due to spurious cross-contamination between samples.

    Article Snippet: Extra-thick fi lter paper (Bio-Rad).

    Techniques: Expressing, Activity Assay